ADH1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. ELISA: 1/5,000-1/20,000. Antibody has been assayed against 1.0 ug of Alcohol dehydrogenase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.
Reactivity	Yeast
Host	Rabbit
Clonality	Polyclonal
Immunogen	Alcohol dehydrogenase from yeast
Specificity	This antibody detects yeast alcohol dehydrogenase. Cross reactivity against alcohol dehydrogenase from other sources may occur but have not been specifically determined. Immunoelectrophoresis gives a single precipitin arc against anti-Biotin, anti-rabbit serum as well as purified and partially purified yeast alcohol dehydrogenase.
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: Biotin State: Lyophilized purified Ig faction Stabilizer: 10 mg/m BSA (immunoglobulin and protease free) Preservative: 0.01% Sodium azide
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P00330
Synonyms	Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, YOL086C, O0947
Reference Data

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