Alkaline phosphatase / ALPI / IAP Rabbit Polyclonal Antibody
CAT#: R1053HRPS
Alkaline phosphatase / ALPI / IAP rabbit polyclonal antibody, HRP, Purified
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CNY 7,688.00
货期*
4周
规格
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Specifications
Product Data | |
Applications | ELISA, WB |
Recommend Dilution | Western Blot: 1/200-1/1,000. ELISA: 1/2,000-1/10,000. Anti Alkaline phosphatase has been assayed against 1.0 µg of Alkaline phosphatase [E.coli] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/3,500 to 1/15,000 of the reconstitution concentration is suggested. |
Reactivity | Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Alkaline phosphatase from calf intestine |
Specificity | This product detects bovine Alkaline phosphatase. Cross reactivity against Alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Alkaline phosphatase [calf intestine]. |
Formulation | 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase |
Reconstitution Method | Restore with 0.1 ml of deionized water (or equivalent). |
Concentration | lot specific |
Purification | Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer |
Conjugation | HRP |
Storage Condition | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Database Link | |
Synonyms | Intestinal alkaline phosphatase |
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