SOD1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: 1/5,000-1/20,000. Western blot: 1/500-1/2,000. This product has been assayed against 1.0 µg of Superoxide Dismutase [bovine erythrocytes] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/2,000 of the reconstitution concentration is suggested.
Reactivity	Bovine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Superoxide Dismutase from Bovine erythrocytes
Specificity	This antibody detects bovine Superoxide Dismutase. Cross reactivity against Superoxide Dismutase from other tissues and species may occur but have not been specifically determined Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified Superoxide Dismutase [bovine erythrocytes].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamycin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 281495 Bovine UniProt ID P00442
Synonyms	SOD-1, CuZn-SOD, CuZnSOD, IPOA, ALS1
Reference Data

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