Ferret IgM (mu chain specific) Goat Polyclonal Antibody

Specifications

Product Data
Recommend Dilution	Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivity	Ferret
Host	Goat
Clonality	Polyclonal
Immunogen	Ferret IgM µ mu chain.
Specificity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Ferret IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Ferret IgM and Ferret Serum. No reaction was observed against Ferret IgG or Ferret IgA. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Ferret heavy or light chain isotypes.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative. Label: FITC State: Lyophilized purified Ig fraction. Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons) Absorption emission: 495 nm / 528 nm Molar radio: 4.0 moles FITC per mole of Goat IgG.
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Immunoaffinity chromatography.
Conjugation	FITC
Storage Condition	Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Synonyms	Ferret Immunoglobulin M
Reference Data

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