Alanine aminotransferase 1 / ALT1 Sheep Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Western blot: 1/1,000-1/5,000. ELISA: 1/10,000-1/40,000. This antibody has been assayed against 1.0 µg of Alanine transaminase [pig heart] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/3,000 of the reconstitution concentration is suggested.
Reactivity	Porcine
Host	Sheep
Clonality	Polyclonal
Immunogen	Alanine transaminase (ALT) from pig heart
Specificity	This antibody detects porcine alanine transaminase. Cross reactivity against alanine transaminase from other sources is unknown. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-sheep serum as well as purified and partially purified alanine transaminase [pig heart].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: HRP State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/m BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!) Label: Horseradish peroxidase
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.  Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Database Link	UniProt ID P13191
Background	Alanine transaminase (ALT) is also referred to as Glutamic pyruvic transaminase (GPT).
Synonyms	GPT, AAT1, GPT1
Reference Data

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