CD11b Rat Monoclonal Antibody [Clone ID: M1/70.15]

Specifications

Product Data
Clone Name	M1/70.15
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Western blot. Flow Cytometry: 1/50-1/200. This product is routinely tested on Mouse peritoneal Macrophages. Immunoprecipitation. Immunofluorescence. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: Clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue. (4)
Reactivity	Human, Mouse, Rabbit
Host	Rat
Clonality	Monoclonal
Immunogen	T cell enriched splenocytes from B10 mice. Spleen cells from immunised DA Rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.
Specificity	This antibody recognises the Murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1). Clone M1/70.15 has been reported to block iC3b binding to its receptor. SM014A is recommended for this purpose.
Formulation	PBS State: Purified State: Liquid purified IgG fraction from tissue culture supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P05555
Background	MAC-1 is a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.
Synonyms	ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1
Reference Data

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