CD11b Rat Monoclonal Antibody [Clone ID: M1/70.15]

Specifications

Product Data
Clone Name	M1/70.15
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Reactivity	Human, Mouse, Rabbit
Host	Rat
Clonality	Monoclonal
Immunogen	T cell enriched splenocytes from B10 mice. Spleen cells from an immunised DA rat were fused with cells of the mouse NS1/1.Ag4.1 myeloma cell line.
Specificity	This antibody recognises the murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1). Clone M1/70.15 has been reported to block iC3b binding to its receptor. SM014A is recommended for this purpose. Clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide, 1% BSA, 5% Sucrose Label: PE State: Lyophilized purified IgG fraction. Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with distilled water.
Purification	Affinity Chromatography on Protein G
Conjugation	PE
Storage Condition	Prior to and following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Database Link	UniProt ID P05555
Background	CD11b (integrin aM subunit) is a 165 kDa type I transmembrane glycoprotein that non-covalently associates with integrin b2 subunit (CD18); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.
Synonyms	ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1
Reference Data

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