Cd4 (non-polymorphic epitope) Rat Monoclonal Antibody [Clone ID: YTS177.9]

Specifications

Product Data
Clone Name	YTS177.9
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/50-1/200 diluted antibody to label 10e6 cells in 100 µl.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse spleen cells. Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
Specificity	SM019LE recognises Murine CD4, a 55kD cell surface antigen expressed by a subset of T lymphocytes. This Low Endotoxin CD4 antibody is reported to block MHC class II dependant T-cell responses in vitro and in vivo and induces tolerance.
Formulation	PBS, pH 7.4 without preservatives. State: Low Endotoxin State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Protein G Affinity chromatography from tissue culture supernatant
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	CD4 antigen
Database Link	Entrez Gene 12504 Mouse UniProt ID P06332
Background	CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin Intracellular ligands: p56Lck CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Synonyms	T-cell surface antigen T4/Leu-3
Reference Data

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