CD45 / LCA (CD45R) Rat Monoclonal Antibody [Clone ID: RA3-6B2]

Specifications

Product Data
Clone Name	RA3-6B2
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/100-1/200 diluted antibody to label 10e6 cells in 100 µl. Immunoprecipitation. Immunohistochemistry on Frozen and Paraffin Sections (PLP fixed tissue-See Ref.3).
Reactivity	Feline, Human, Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse pre-B tumour cells (RAW112). Spleen cells from immunised Lewis rats were fused with cells of the rat S194/5 XX0.BU-1 myeloma cell line.
Specificity	This antibody reacts with CD45R a form of the CD45 antigen expressed by B cells and lytically active subsets of NK cells and non-MHC restricted CTLs. Clone RA3-6B2 immunoprecipitates the high molecular weight form of CD45 (220kD).
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	protein tyrosine phosphatase, receptor type, C
Database Link	Entrez Gene 19264 Mouse UniProt ID P06800
Background	CD45R is a member of the protein tyrosine phosphatase (PTP) family and a major cell surface glycoprotein. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R represents a restricted form of the CD45 family which primarily recognizes only cells of B lineage from proB cell through mature B lymphocytes and, prior to the availability of anti CD19 MAbs, was commonly used as a pan B cell marker. It also reacts with certain activated T cells, as well as non MHC restricted lytically active lymphokine activated killer (LAK) cells. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T and B cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. CD45R also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data

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