CD161 (KLRB1) Mouse Monoclonal Antibody [Clone ID: PK136]

Specifications

Product Data
Clone Name	PK136
Applications	CT, FC, IP
Recommend Dilution	Flow Cytometry: Use 10 µl of 5-10 µg/ml diluted antibody to label 1 x 10e6 cells in 100 µl. Immunoprecipitation. Cytotoxic Assays: 1-10 µg/ml.
Reactivity	Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Spleen and bone marrow cells from CE mice. Spleen cells from immunised (C3H x BALB/c) FI Hybrid were fused with cells of the Sp2/0 - Ag14 myeloma cell line.
Specificity	This antibody (clone PK136) recognises the NK1.1 cell surface antigen. Although previously thought to recognise only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory. This antibody is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. The antibody has also been used for in vivo depletion of NK cells and in vitro activation of NK cells.  Negative Species: Human, Rat.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	killer cell lectin like receptor B1
Database Link	Entrez Gene 3820 Human UniProt ID Q12918
Background	NK1.1 is a cell surface glycoprotein encoded by members of the NKR-P1 gene family. In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc.
Synonyms	HNKR-P1a, CLEC5B, NKRP1A
Reference Data
Protein Families	Transmembrane

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