CD3E Mouse Monoclonal Antibody [Clone ID: UCHT1]

Specifications

Product Data
Clone Name	UCHT1
Applications	FC, FN, IHC, IP
Recommend Dilution	Flow Cytometry: The epitope for UCHT1, CD3 is resistant to fixation. For purposes where pre-fixed cells are stained, this antibody is recommended. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Functional Assay: The immobilized UCHT1 antibody initiates a signaling pathway resulting in T cell activation and proliferation.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Human thymocytes followed by Sezary T cells
Specificity	This antibody recognizes the CD3 antigen of the TCR/CD3 complex on mature Human T cells. The UCHT1 antibody reacts with the epsilon chain of the CD3 complex.
Formulation	Azide Free phosphate buffered saline (PBS), pH~7.4 and 0.2 μm filter sterilized State: Azide Free State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Protein-A Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD3e molecule
Database Link	Entrez Gene 699467 MonkeyEntrez Gene 916 Human UniProt ID P07766
Background	CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Synonyms	T3/Leu-4
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.