CD49b (ITGA2) Mouse Monoclonal Antibody [Clone ID: AK7]

Specifications

Product Data
Clone Name	AK7
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat CD49b antibody to label 10e6 cells or 100 µl human whole blood.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Specificity	Recognizes the Integrin alpha 2 subunit (CD49b), which is a 160kD glycoprotein that non-covalently associates with the 130kD integrin beta1 subunit to form the VLA-2 complex.
Formulation	PBS, pH 7.2 with 0.09% Sodium Azide as preservative, 1% BSA and 5% Sucrose. Label: PE State: Lyophilized purified IgG fraction Label: R. Phycoerythrin (RPE)
Reconstitution Method	Restore with 1.0 ml distilled water.
Purification	Affinity Chromatography on Protein G
Conjugation	PE
Storage Condition	Prior to and Following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	integrin subunit alpha 2
Database Link	Entrez Gene 3673 Human UniProt ID P17301
Background	Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. They are responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. CD49b is a 160kD glycoprotein that non-covalently associates with the 130kD integrin beta 1 subunit to form the VLA-2 complex. CD49b is expressed by platelets, long term cultivated T cells, approximately 50% of monocytes and most adherent cell lines.
Synonyms	Integrin alpha-2, ITGA-2, VLA-2 alpha, VLA2, GPIa, Collagen Receptor
Note	Fusion Partners: Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
Reference Data

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