HLA Class I ABC Mouse Monoclonal Antibody [Clone ID: W6/32]

Specifications

Product Data
Clone Name	W6/32
Applications	ELISA, FC, FN, IF, IHC, IP
Recommend Dilution	ELISA. Immunoprecipitation (Ref 1). Functional Assays. Immunofluoresecnce. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells in 100 µl. This product is routinely tested in Flow Cytometry on Human peripheral blood lymphocytes. Immunohistochemistry on Frozen Sections (See Ref 6). Positive Control Tissue: Tonsil. Not suitable for Immunohistochemistry on Paraffin Sections.
Reactivity	Bovine, Chimpanzee, Feline, Human, Macaque, Monkey, Primate, Baboon
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified Human tonsil lymphocyte membranes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line
Specificity	SM1222LE recognises an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognises a conformational epitope, reacting with HLA class I alpha-3 and alpha-2 domains (Ref 7). MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells.
Formulation	PBS without preservatives or stabilizers. Endotoxin level: Less than 0.01 EU/µg State: Low Endotoxin State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at -20°C only. Avoid repeated freezing and thawing.
Synonyms	MHC class I ABC, HLA Class 2 ABC
Reference Data

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