Cytokeratin 14 (KRT14) Mouse Monoclonal Antibody [Clone ID: LL002]

Specifications

Product Data
Clone Name	LL002
Applications	FC, IF, IHC, WB
Recommend Dilution	Western Blot. Immunofluorescence. Flow Cytometry: Use 10 µl of 1/100 diluted antibody to label 106 cells in 100 µl (Membrane permeabilisation is required). Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/200. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer is recommended for this purpose. Enzyme digestion is not recommended. Recommended Positive Control: Skin, tonsil or squamous cell carcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Last 15 C-terminal residues of Human Cytokeratin 14 conjugated to Thyroglobulin.
Specificity	This antibody recognises Cytokeratin 14, a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express Cytokeratin 14.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Ion Exchange Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 14
Database Link	Entrez Gene 3861 Human UniProt ID P02533
Background	Cytokeratin is a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express cytokeratin 14. Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer.
Synonyms	Cytokeratin-14, CK14, Keratin 14, K14, KRT14
Reference Data

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