Factor I (CFI) Mouse Monoclonal Antibody [Clone ID: OX-21]

Specifications

Product Data
Clone Name	OX-21
Applications	ELISA, FC, IHC, IP, R, WB
Recommend Dilution	ELISA.  Titre: 1/40 000 (plates coated with 2 µg/ml purified Factor I and HRPO Goat anti-Mouse IgG secondary antibody). Western Blot.   Flow Cytometry. Immunoprecipitation. Immunohistochemistry on Paraffin Sections (20 µg/ml).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified Human Complement Factor 1.   Spleen cells from immunised FI cross DBA/2 x Balb/c mice were fused with cells of the NS-1 mouse myeloma cell line.
Specificity	This monoclonal antibody clone OX-21 recognizes Human Complement Protein Factor I. Factor I has two polypeptide chains, 56kD and 38kD. This antibody detects only the 56kD chain.  This clone has been reported not to cross react with Rat.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	complement factor I
Database Link	Entrez Gene 3426 Human UniProt ID P05156
Background	Factor I is a plasma protein of molecular weight 88 kDa, consisting of two disulphide linked chains of 50 and 38 kDa. Factor I is a serine protease with a high degree of specificity for C3b and C4b. It has a requirement for protein cofactors for cleavage of these complement proteins, Factor H, CR1 or MCP are required for C3b cleavage and C4bp or CR1 for C4b cleavage.
Synonyms	CFI, IF, KAF, AHUS3, C3BINA, C3b-INA
Reference Data
Protein Families	Druggable Genome, Protease, Secreted Protein
Protein Pathways	Complement and coagulation cascades

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