PKC alpha (PRKCA) Mouse Monoclonal Antibody [Clone ID: 133]

Specifications

Product Data
Clone Name	133
Applications	IHC, WB
Recommend Dilution	Western Blot: 1/10-1/500. Immunohistochemistry on Frozen Sections: Neat-1/10.
Reactivity	Human, Mammalian
Host	Mouse
Clonality	Monoclonal
Immunogen	Protein Kinase C alpha (PKC alpha). Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line.
Specificity	PKC alpha antibody (Cat#SM1436) recognises the alpha isoform of Protein Kinase C. It recognises the sequence PQFVHPILQSAV at the C terminus of PKCa.
Formulation	State: Supernatant State: Liquid Tissue Culture Supernatant containing 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	protein kinase C alpha
Database Link	Entrez Gene 5578 Human UniProt ID P17252
Background	Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide dependent kinase 1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation.
Synonyms	Protein kinase C alpha type, PKC-alpha, PKC alpha, PKC-A, PKCA, PRKACA
Reference Data

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