TNF alpha (TNF) Rat Monoclonal Antibody [Clone ID: MP9-20A4]

Specifications

Product Data
Clone Name	MP9-20A4
Applications	ELISA, FC, IHC
Recommend Dilution	Flow Cytometry: Membrane permeabilisation is required for this application. ELISA: 5µg/ml - 10µg/ml. Immunohistochemistry on frozen sections.
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Specificity	This antibody recognises Tumour Necrosis Factor - alpha (TNF alpha), also known as TNFSF2. Clone MP9 - 20A4 is reported to act as a blocking antibody. We recommend the use of SM1464A for functional studies.
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tumor necrosis factor
Database Link	Entrez Gene 7124 Human UniProt ID P01375
Background	This 17kDa cytokine is expressed by activated macrophages, monocytes, neutrophils, T cells and NK cells.
Synonyms	TNF, TNF-a, TNFA, TNFSF2, Cachectin
Reference Data

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