CD81 / TAPA1 Hamster Monoclonal Antibody [Clone ID: Eat2]

Specifications

Product Data
Clone Name	Eat2
Applications	ELISA, FC, IHC, IP, WB
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl). Immunoprecipitation. Immunohistochemistry on Frozen Sections. ELISA. Western Blot. Not suitable for Paraffin Embedded Sections.
Reactivity	Mouse, Rat
Host	Hamster
Clonality	Monoclonal
Immunogen	38C13, murine B cell line
Specificity	This antibody recognises CD81, a 26kD cell surface glycoprotein.
Formulation	PBS, pH 7.4 with 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	CD81 antigen
Database Link	Entrez Gene 12520 Mouse UniProt ID P35762
Background	In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. Eat 2 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation. Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding.
Synonyms	S5.7; TAPA-1; TAPA1; Tetraspanin-28; Tspan-28; TSPAN28
Reference Data

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