Cd68 Rat Monoclonal Antibody [Clone ID: FA-11]
CAT#: SM1550PS
Cd68 rat monoclonal antibody, clone FA-11, Purified
Size: 250 ug
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CNY 5,434.00
货期*
5周
规格
Cited in 4 publications. |
Specifications
Product Data | |
Clone Name | FA-11 |
Applications | FC, IF, IHC, IP, WB |
Recommend Dilution | Western Blot: Non-reducing conditions recommended. Immunoprecipiation. Immunofluorescence. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells in 100 µl (Membrane permeabilization is required). Clone FA-11 can be used in Flow Cytometry to detect intracellular CD68, following permeabilization, and can detect surface macrosialin at low levels in resident Mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections. Rat anti Mouse CD68 antibody, Clone FA-11, has been used in many mouse models for the identification of CD68 in immunohistochemical studies, using both frozen and paraffin-embedded tissues, See Masaki, T. et al. and Devey, L. et al. as examples. -This product may require antigen retrieval using heat treatment prior to staining of paraffin sections. Either sodium citrate buffer or Tris/EDTA buffer may be used for this purpose. See Martin-Manso for details. -Staining has also been achieved without antigen retrieval, See Lu et al. for details. |
Reactivity | Mouse |
Host | Rat |
Clonality | Monoclonal |
Immunogen | Purified Concanavalin A acceptor glycoprotein from P815 cell line. |
Specificity | Clone FA-11 recognizes mouse macrosialin, a heavily glycosylated transmembrane protein and murine homolog of human CD68, which is classified as a unique scavenger receptor (ScR) family member, due to the presence of a lysosome associated membrane protein (LAMP)-like domain. |
Formulation | PBS State: Purified State: Liquid purfied IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein G |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Gene Name | CD68 antigen |
Database Link | |
Background | CD68 is considered a pan macrophage marker, predominantly expressed on the intracellular lysosomes of tissue macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes. CD68 is expressed by many tumor types including some B cell lymphomas, blastic NK lymphomas, melanomas, granulocytic (myeloid) sarcomas, hairy cell leukemias, and renal, urinary and pancreatic tumors, and can be used in cancer studies to demonstrate the presence/localization of macrophages. |
Synonyms | Gp110, Macrosialin, Macrophage marker |
Reference Data |
Citations (4)
The use of this Antibodies has been cited in the following citations: |
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Resveratrol and Its Analogue 4,4′-Dihydroxy-trans-stilbene Inhibit Lewis Lung Carcinoma Growth In Vivo through Apoptosis, Autophagy and Modulation of the Tumour Microenvironment in a Murine Model
,null,
Biomedicines
,PubMed ID 35892684
[Cd68]
|
Successful use of mRNA-nucleofection for overexpression of interleukin-10 in murine monocytes/macrophages for anti-inflammatory therapy in a murine model of autoimmune myocarditis
,Zimmermann O, Homann JM, Bangert A, M|ller AM, Hristov G, Goeser S, Wiehe JM, Zittrich S, Rottbauer W, Torzewski J, Pfitzer G, Katus HA, Kaya Z,
J Am Heart Assoc
,PubMed ID 23316321
[CD68]
|
SIGNR1-negative red pulp macrophages protect against acute streptococcal sepsis after Leishmania donovani-induced loss of marginal zone macrophages
,Kirby AC, Beattie L, Maroof A, van Rooijen N, Kaye PM,
Am. J. Pathol.
,PubMed ID 19644016
[CD68]
|
Myeloid-Specific Deletion of Diacylglycerol Lipase α Inhibits Atherogenesis in ApoE-Deficient Mice
,Jehle J, Hoyer FF, SchC6ne B, Pfeifer P, Schild K, Jenniches I, Bindila L, Lutz B, LC |
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