Estrogen Receptor 1 (ESR1) Mouse Monoclonal Antibody [Clone ID: 6F11]

Specifications

Product Data
Clone Name	6F11
Applications	IHC, WB
Recommend Dilution	Western Blot: 1/50-1/100. Immunohistochemistry on Frozen Sections: 1/40-1/60. Zambonis fixative recommended for best results. Immunohistochemistry on Paraffin Sections: 1/40-1/80. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections; Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Breast carcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human estrogen receptor (alpha form). Spleen cells from immunised mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line.
Specificity	This antibody recognises the Estrogen Receptor alpha chain. The Oestrogen Receptor status of breast cancer tissues is an important parameter in determining prognosis and response to therapy.
Formulation	State: Supernatant State: Lyophilized Tissue Culture Supernatant containing 0.09% Sodium Azide
Reconstitution Method	Restore with 1 ml distilled water
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C Prior to reconstitution and at -20°C Following reconstitution. Avoid repeated freezing and thawing.
Gene Name	estrogen receptor 1
Database Link	Entrez Gene 2099 Human UniProt ID P03372
Background	Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies
Synonyms	ER alpha, Estradiol receptor, ESR1, ESR, NR3A1
Reference Data

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