Mrc1 Rat Monoclonal Antibody [Clone ID: MR5D3]

Specifications

Product Data
Clone Name	MR5D3
Applications	FC, IF, IHC, IP
Recommend Dilution	Immunoprecipitation. Immunofluorescence. Immunohistochemistry on Frozen Sections. Flow Cytometry: Use 10 µl of 1/10-1/20 diluted antibody to label 106 cell in 100 ul. CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Has been reported to work in Western Blot (Ref.1)
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Chimaeric CRD4-7-Fc protein. Spleen cells from immunised Fischer rats were fused with cells of the Y3 myeloma cell line.
Specificity	This antibody recognises the Mannose Receptor, a 175kD type 1 membrane protein that is also known as CD206. Clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands (Zamze et al. 2002). Clone MR5D3 has also been shown to work in Western Blot (Martinez-Pomares et al. 2003 and Su et al. 2005).
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	mannose receptor, C type 1
Database Link	Entrez Gene 17533 Mouse UniProt ID Q61830
Background	CD206 is a transmembrane protein and member of the multilectin family of pattern recognition receptors (PRRs), which mediates phagocytosis and endocytosis, and is modulated by a range of factors including cytokines, immunoglobulin receptors and pathogens. CD206 has two extracellular lectin binding sites: the cysteine-rich domain binds to sulphated sugars of glycoproteins such as thyroid stimulating hormone, whilst the recognition of mannose and fucose occurs through carbohydrate recognition domains (CRDs). The widespread distribution and recognition of a wide range of antigens by macrophages, plays a key role in both innate and adaptive immunity, with CD206 able to bind to both gram-negative and gram-positive bacteria, mycobacterium, yeasts, fungi and parasites. The expression of CD206 is not restricted to macrophages, but is also present on immature dendritic cells (DCs), endothelial cells, kidney mesangial cells, retinal pigment epithelium and tracheal smooth muscle cells.
Synonyms	Macrophage mannose receptor, CLEC13D, CLEC13DL, MRC1L1
Reference Data

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