CD8A Mouse Monoclonal Antibody [Clone ID: CVS8]

Specifications

Product Data
Clone Name	CVS8
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/25-1/200 diluted CD8 antibody to label 106 cells in 100 µl. Immunoprecipitation. Immunohistochemistry on Frozen Sections.
Reactivity	Donkey, Equine
Host	Mouse
Clonality	Monoclonal
Immunogen	Equine PBMC. Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Specificity	This monoclonal CVS8 antibody recognises the Equine homologue of the Human CD8 cell surface antigen which is expressed by a subset of T lymphocytes. A study undertaken using CVS8 to identify CD8 on several wild african equid species indicates that the CVS8 clone recognises Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra) (Ibrahim, S, 2007). Negatives Species: Zebra.
Formulation	PBS State: Purified State: Liquid purified IgG fraction from tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD8a molecule
Database Link	UniProt ID P01732
Background	The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
Synonyms	CD8 alpha chain, CD8A, MAL
Reference Data

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