Anpep Rat Monoclonal Antibody [Clone ID: ER-BMDM1]

Specifications

Product Data
Clone Name	ER-BMDM1
Applications	FC, IHC
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/10-1/50 diluted CD13 antibody to label 106 cells in 100 µl. Immunohistochemistry on Frozen Sections.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Balb/c bone marrow derived Macrophages. Cells from immunised rats were fused with cells of the rat Y3-Ag1.2.3 myeloma cell line.
Specificity	This antibody Clone ER-BMDM1 Cat.-No SM2298P recognizes the Murine homologue of Human CD13, a 160kD cell surface antigen with Aminopeptidase N activity.
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	alanyl (membrane) aminopeptidase
Database Link	Entrez Gene 16790 Mouse UniProt ID P97449
Background	In steady state mice, the CD13 antigen is expressed primarily by dendritic cell and mature macrophage subpopulations. The level of CD13 antigen expression increases with maturation, after the monocytic stage. Bone marrow cells and peripheral blood monocytes are reported to be CD13 negative while thioglycollate-elicited macrophages express high levels of CD13. Dendritic cells, interdigitating dendritic cells, veiled cells and Langerhans cells all express CD13, which is reported to be involved in the development of immature dendritic cells from monocytes.
Synonyms	Aminopeptidase N, ANPEP, APN, PEPN
Reference Data

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