Anpep Rat Monoclonal Antibody [Clone ID: ER-BMDM1]

Specifications

Product Data
Clone Name	ER-BMDM1
Applications	FC, IHC
Recommend Dilution	Flow Cytometry. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections. Typical Starting working Dilution: 1/50.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Specificity	This antibody Clone ER-BMDM1 Cat.-No SM2298PS is directed against a 160-kDa cell membrane-associated antigen with aminopeptidase N activity. The antigen recognized by the mocnoclonal antibody ER-BMDM1 is present on various Mouse macrophage and dendritic cell subpopulations as well as on microvillous epithelia. The ER-BMDM1 antigen is expressed at increasing levels upon maturation of macrophages. Therefore the monoclonal antibody ER-BMDM1 is a useful marker for the identification of Aminopeptidase N positive macrophages and dendritic cells in Mouse, the expression of the ER-BMDM1 antigen arises after the monocytic stage of differentation: bone marrow cells and peripheral blood monocytes are ER-BMDM1 negative, whereas mature macrophages express the ER-BMDM1 antigen. A subpopulation of dendrocytes and their in vitro equivalents, the dendritic cells, are also ER-BMDM1 positive.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	alanyl (membrane) aminopeptidase
Database Link	Entrez Gene 16790 Mouse UniProt ID P97449
Background	Aminopeptidase N (APN) is also known as Alanyl Aminopeptidase (ANPEP), CD13 antigen or membrane protein p161. The protein consists of a short cyplasmic tail, a transmembrane region, a Ser/Thr region, and a zinc metalloproteinase domain. Widely expressed in many cells, tissues, and species, aminopeptidase N cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. It has putative involvement in several biological processes including antigen processing and presentation, cell adhesion, tumor invasion and metastasis and neurotransmitter degradation.
Synonyms	Aminopeptidase N, ANPEP, APN, PEPN
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.