CD161 (KLRB1) Mouse Monoclonal Antibody [Clone ID: 10/78]

Specifications

Product Data
Clone Name	10/78
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry: 1/50-1/100; use 10ul of the suggested working dilution to label 10e6 cells in 100 ul. Imunoprecipitation. Immunohistochemistry on frozen sections.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified splenic NK cells from the LEW rat strain.
Specificity	This antibody recognises the rat CD161 protein (also known as NKR-PI) a 60kD glycoprotein expressed on rat NK cells and T cell subpopulations. This antibody competes with clone 3.2.3 for binding to antigen. We recommend the use of SM293A use in functional studies. Clone R29 is reported as being suitable for Western blotting. We recommend the use of SM1383R or this application.
Formulation	PBS, pH 7.4 with 0.09% sodium azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Protein G affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles.
Gene Name	killer cell lectin like receptor B1
Database Link	UniProt ID Q12918
Background	Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 (CD161) protein contains an extracellular domain with several motifs characteristic of C type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein is classified as a type II membrane protein because it has an external C terminus. In mouse the NKRP1 family has three members, NKRP1A, B and C, whilst in human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, b and c. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory.
Synonyms	HNKR-P1a, CLEC5B, NKRP1A
Reference Data

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