CD14 Mouse Monoclonal Antibody [Clone ID: MEM-18]

Specifications

Product Data
Clone Name	MEM-18
Applications	ELISA, FC, FN, IP, WB
Recommend Dilution	Flow Cytometry: 4 μg/ml. Immunoprecipitation. Western blot (Non-reducing conditions). ELISA: The antibody MEM-18 has been tested as the detection antibody in a sandwich ELISA for analysis of Human CD14 in combination with SM3008P (Clone B-A8). Functional Assay: The antibody MEM-18 completely blocks binding of fluorescein (FITC) labeled bacterial LPS to the monocyte surface and it also blocks the binding of CD14 to the extracellular TLR2 domain.
Reactivity	Human, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Crude mixture of Human urinary proteins precipitated by Ammonium Sulphate from the urine of a patient suffering from proteinuria
Specificity	The antibody reacts with CD14, a 53-55 kDa GPI(glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes, also expressed by most tissue macrophages. In Human, the epitope recognized by MEM-18 is located between amino acids 57-64.
Formulation	Phosphate buffered saline (PBS), pH~7.4 with 15 mM Sodium Azide as preservative State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD14 molecule
Database Link	Entrez Gene 929 Human UniProt ID P08571
Background	CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor; it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Synonyms	CD14
Reference Data

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