CD63 Mouse Monoclonal Antibody [Clone ID: MEM-259]

Specifications

Product Data
Clone Name	MEM-259
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry: 2 µg/ml (Detection of activated Platelets, Neutrophils and Basophils). Immunoprecipitation. Western blot (Non-Reducing Conditions). Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Positive tissue: Spleen.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	HPB-ALL T cell line
Specificity	The antibody reacts with CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.
Formulation	PBS, pH~7.4 State: Purified State: Liquid purified Ig fraction from hybridoma culture supernatant (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD63 molecule
Database Link	Entrez Gene 967 Human UniProt ID P08962
Background	CD63, a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.
Synonyms	OMA81H, Granulophysin, Tetraspanin-30, MLA1, TSPAN30, ME491
Reference Data

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