LAT Mouse Monoclonal Antibody [Clone ID: LAT-01]

Specifications

Product Data
Clone Name	LAT-01
Applications	FC, IP, WB
Recommend Dilution	Immunoprecipitation: Tyrosine-phosphorylated form of LAT is not optimally recognized. Flow cytometry: Recommended dilution: 1-4 µg/ml, intracellular staining. Tyrosine-phosphorylated form of LAT is not optimally recognized. Western blotting: Recommended dilution: 1-2 μg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmic domain of human LAT
Specificity	The antibody reacts with Linker for activation of T cells (LAT), a 36-38 kDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein A affinity chromatography; purity: > 95 % (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	linker for activation of T-cells
Database Link	Entrez Gene 27040 Human UniProt ID O43561
Background	LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FceRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.
Synonyms	pp36, p36-38
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway

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