NTAL (LAT2) Mouse Monoclonal Antibody [Clone ID: NAP-07]
CAT#: SM3122P
NTAL (LAT2) mouse monoclonal antibody, clone NAP-07, Purified
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CNY 3,810.00
货期*
5周
规格
Cited in 3 publications. |
Specifications
Product Data | |
Clone Name | NAP-07 |
Applications | FC, IF, IP, WB |
Recommend Dilution | Flow Cytometry: Positive tissue: RAJI Human lymphoma cell line. RAMOS Human lymphoma cell line Immunoprecipitation. Western Blot: 2 µg/ml, incubation 60 min. Positive Control: RAMOS Human lymphoma cell line. Negative Control: HeLa Human cervix carcinoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12% separating SDS-PAGE gel. Immunocytochemistry. |
Reactivity | Human, Mouse |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Recombinant cytoplasmic domain (aa 91-244) of human NTAL |
Specificity | The antibody reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells. |
Formulation | Phosphate buffered saline (PBS) with 15 mM Sodium Azide, pH~7.4 State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein A |
Conjugation | Unconjugated |
Storage Condition | Store undiluted at 2-8°C. DO NOT FREEZE! |
Gene Name | linker for activation of T-cells family member 2 |
Database Link | |
Background | NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function. |
Synonyms | LAB, NTAL, WBS15, WBSCR5, HSPC046 |
Reference Data |
Citations (3)
The use of this Antibodies has been cited in the following citations: |
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Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL
,Volna P, Lebduska P, Draberova L, Smmova S, Heneberg P, Boubelmk M, Bugajev V, Malissen B, Wilson BS, Horejsm V, Malissen M, Draber P,
J. Exp. Med.
,PubMed ID 15477348
[LAT2]
|
NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation
,Tkaczyk C, Horejsi V, Iwaki S, Draber P, Samelson LE, Satterthwaite AB, Nahm DH, Metcalfe DD, Gilfillan AM,
Blood
,PubMed ID 15010370
[LAT2]
|
Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling
,Brdicka T, Imrich M, Angelisova P, Brdickova N, Horvath O, Spicka J, Hilgert I, Luskova P, Draber P, Novak P, Engels N, Wienands J, Simeoni L, Osterreicher J, Aguado E, Malissen M, Schraven B, Horejsm V,
J. Exp. Med.
,PubMed ID 12486104
[LAT2]
|
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