LIME (LIME1) Mouse Monoclonal Antibody [Clone ID: LIME10]

Specifications

Product Data
Clone Name	LIME10
Applications	FC, IHC, WB
Recommend Dilution	Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml. Western blotting: Reducing conditions; recommended dilution: 1 μg/ml. Flow cytometry: Recommended dilution: 1-5 μg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin
Specificity	The antibody reacts with the cytoplasmic domain of LIME, a 31 kDa Lck-interacting transmembrane adaptor expressed by T cells.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein A affinity chromatography; purity: > 95% (by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	Lck interacting transmembrane adaptor 1
Database Link	Entrez Gene 54923 Human UniProt ID Q9H400
Background	LIME (Lck-interacting molecule) is a 31 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
Synonyms	LIME1, LIME, LIME-1, Lck-interacting membrane protein, Lck-interacting molecule, Lck-interacting transmembrane adapter 1
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.