CD5 Mouse Monoclonal Antibody [Clone ID: CVS5]

Specifications

Product Data
Clone Name	CVS5
Applications	FC
Recommend Dilution	Flow cytometry: Neat, use 10µl to label 10e6 cells in 100µl.
Reactivity	Equine
Host	Mouse
Clonality	Monoclonal
Immunogen	Equine leucocytes.
Specificity	CVS5 recognises the equine CD5 antigen with an approximate molecular weight of 69kDa. Equine CD5 is expressed on the majority of T lymphocytes, in addition is has been reported that equine CD5 may also be detected at very low levels on B cells and granulocyctes.
Formulation	Containing 0.09% Sodium azide, 1% BSA and 5% Sucrose Label: PE State: Purified State: Lyophilised purified IgG Label: R. Phycoerythrin
Reconstitution Method	Restore with 1 ml distilled water.
Purification	Affinity chromatography on Protein G
Conjugation	PE
Storage Condition	Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Database Link	UniProt ID F6PYA8
Background	CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.
Synonyms	CD5, LEU1
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.