Ccl24 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect mEotaxin-2 (using 100 μl/well antibody solution) a concentration of ~1.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 2000-4000 pg/well of recombinant mEotaxin-2. Sandwich ELISA: To detect mEotaxin-2 (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This In conjunction with Polyclonal Anti-Murine Eotaxin-2 as a capture antibody, it allows the detection of at least 2000-4000 pg/well of recombinant mEotaxin-2. Western blot: To detect mEotaxin-2 this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEotaxin-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Mouse
Host	Goat
Clonality	Polyclonal
Immunogen	E. coli derived, 10.3 kDa highly pure (> 98%) recombinant Murine Eotaxin-2
Specificity	This antibody detects Eotaxin 2
Formulation	PBS, pH 7.2 Label: Biotin State: Sterile filtered lyophilized Ig fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/ml.
Purification	Affinity chromatography
Conjugation	Biotin
Storage Condition	Upon receipt, store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeated freezing and thawing.
Gene Name	chemokine (C-C motif) ligand 24
Database Link	Entrez Gene 56221 Mouse UniProt ID Q9JKC0
Background	Eotaxin 2 is a member of the CC chemokine family, based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin 2 cDNA encodes a 119 amino acid residue precursor protein with a 26 amino acid residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N glycosylation site. Mature human eotaxin 2 has a predicted molecular mass of approximately 10.6 kDa. Compared to other CC chemokines, eotaxin 2 exhibits 40 %, 42 %, and 39 % amino acid identity to MCP3, MIP1 alpha, and eotaxin, respectively. Human CC chemokine eotaxin 2 maps to chromosome 7q11.23. Both eotaxin and eotaxin 2 activate and attract eosinophils and basophils. A receptor for human eotaxin has been identified and found to be the third numbered receptor in the C-C chemokine subfamily of receptors (CCR3. On eosinophils, the effects of eotaxin 2 is inhibited by an CCR3 antibody and cross-desensitized by eotaxin and MCP4, suggesting that all three CC chemokines act through CCR3. Eotaxin 2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant eotaxin 2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin 2 inhibits colony formation in myleloid progenitor cells.
Synonyms	C-C motif chemokine 24, Small-inducible cytokine A24, MPIF2, MPIF-2, SCYA24, CK-beta-6
Reference Data

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