SLP76 (LCP2) Rabbit Polyclonal Antibody

CAT#: SP3000P

SLP76 (LCP2) rabbit polyclonal antibody, Purified



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CNY 3,810.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Applications IHC, WB
Recommend Dilution Western blot.
Positive control: JURKAT human T cell leukemia cell lysate.
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
Application note: Reducing conditions.
Immunohistochemistry on paraffin sections: 10 μg/ml.
Positive tissue: thymus.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen A fusion protein representing amino acids 216-434 of human SLP-76
Specificity The antibody reacts with SLP76, a 76 kDa cytosolic adaptor protein that is involved in signaling of various hematopoietic cells, such as T cells, mast cells or neutrophils; in B cells, however, it is replaced by SLP65.
Formulation Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
State: Purified
State: Liquid Ig fraction
Concentration lot specific
Purification By precipitation methods; purity: > 95% (by SDS-PAGE)
Conjugation Unconjugated
Storage Condition Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name lymphocyte cytosolic protein 2
Background SLP76 (SH2 domain-containing leukocyte protein of 76 kDa) is a cytosolic adaptor protein which translocates to the plasma mambrane and is involved in multiple signaling pathways in T cells, mast cells, neutrophils and platelets; B cells express its analog SLP65/BLNK (B cell linker protein). SLP76 is phosphorylated by Syk-family and Tec-family tyrosine kinases and couples them to the phosphorylation and activation of PLC-g. Via Gads or Grb2, SLP76 also associates with LAT adaptor by involvement of SLP76 proline-rich region. The SH2 domain of SLP76 has been identified as the region involved in binding the serine/threonine kinase HPK1. HPK1 may act as both a positive and a negative regulator by promoting the Jnk-mitogen activated protein kinase (MAPK) pathway and inhibiting the pathway leading to AP-1 activation.
Synonyms LCP2, Lymphocyte cytosolic protein 2, SLP-76 tyrosine phosphoprotein, SLP76
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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