PAG (PAG1) (97-432) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, IP, WB
Recommend Dilution	Western Blotting: 1-2 μg/ml. Immunoprecipitation. Immunohistochemistry on Paraffin Sections. Positive tissue: tonsil, colon germinal center.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant intracellular fragment (aa 97-432) of human Cbp/PAG
Specificity	The antibody recognizes Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
Formulation	Phosphate buffered saline (PBS), pH~7.4 containing 15 mM Sodium Azide as preservative. State: Purified State: Liquid purified Ig fraction (>95% pure by SDS-PAGE).
Concentration	lot specific
Purification	Precipitation Methods.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	phosphoprotein membrane anchor with glycosphingolipid microdomains 1
Database Link	Entrez Gene 55824 Human UniProt ID Q9NWQ8
Background	PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
Synonyms	PAG, CBP
Reference Data

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