SARS-CoV-2/SARS-CoV Spike Protein Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	E, WB
Recommend Dilution	ELISA: the antibody will detect 2 ng of free peptide at 1 μg/ml. SARS-CoV-2/SARS-CoV Spike antibody can be used for the detection of SARS-CoV-2/SARS-CoV Spike protein in ELISA and in Western Blot.
Reactivity	SARS-CoV, SARS-CoV-2
Host	Rabbit
Clonality	Polyclonal
Immunogen	SARS-CoV-2 Spike protein antibody was raised against a peptide corresponding to 17 amino acids within aa 410-460 near the center of SARS-CoV Spike glycoprotein. Predicted reactivity based on immunogen sequence: SARS-CoV-2 Spike protein: (identity 65%, homology 88%). Has been shown to react with SARS-CoV-2 protein (see QC images).
Formulation	PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Database Link	UniProt ID P59594
Background	SARS Spike Antibody: A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin-converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2.
Reference Data

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