APE1 (APEX1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	Block/Neutralize, ChIP, FC, ICC/IF, IHC, IP, Simple Western, WB
Recommend Dilution	Chromatin Immunoprecipitation (ChIP): 1:10-1:500, Immunohistochemistry: 1:100, Immunocytochemistry/ Immunofluorescence: 1:50-1:200, Immunoprecipitation: 7 ug/ml, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:100, Immunohistochemistry-Frozen: 1:100, Simple Western: 1:12.5, Flow Cytometry, Immunohistochemistry Free-Floating, Block/Neutralize
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Affinity purified human Apurinic/Apyrimidinic Endonuclease (APE/ref-1) fusion protein.
Formulation	Tris-glycine, 150mM NaCl and 0.05% sodium azide
Purification	Affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	apurinic/apyrimidinic endodeoxyribonuclease 1
Database Link	NP_001632 Entrez Gene 11792 MouseEntrez Gene 79116 RatEntrez Gene 328 Human UniProt ID P27695
Background	The mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is responsible for the repair of AP sites in DNA. In addition, this enzyme has been shown to function as a redox factor facilitating the DNA binding capability of FOS, JUN, NfkB, HIF-1a as well as other transcription factors. Recently, APE has also been shown to control p53 activity through redox alteration. APE is also linked to apoptosis, associated with thioredoxin, and altered levels of APE have been found in some cancers. APE appears to form a unique link between the DNA base excision pathway, oxidative signaling, transcription regulation, cancer and cell-cycle control.
Synonyms	APE; APE1; APEN; APEX; APX; HAP1; REF1
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency, Transcription Factors
Protein Pathways	Base excision repair

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