HIF1 beta (ARNT) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ChIP, ICC/IF, IHC, Immunoblotting, IP, Simple Western, WB
Recommend Dilution	Immunohistochemistry: 1:150, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation: 1:10-1:500, Western Blot: 1:2000, Immunohistochemistry-Paraffin: 1:150, Simple Western: 1:1000, Gel Supershift Assay, Chromatin Immunoprecipitation (ChIP): 1:10-1:500, Immunoblotting
Reactivity	Human, Bovine, Mouse, Rat, Sheep, Ferret
Host	Rabbit
Clonality	Polyclonal
Immunogen	Fusion protein to human HIF-1 beta. Containing a.a. 496-789.
Formulation	0.02% sodium azide
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	aryl hydrocarbon receptor nuclear translocator
Database Link	NP_001659 Entrez Gene 11863 MouseEntrez Gene 25242 RatEntrez Gene 405 Human UniProt ID P27540
Background	Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. (1,5,6)
Synonyms	bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; TANGO
Reference Data
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Pathways in cancer, Renal cell carcinoma

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