Wilms Tumor Protein (WT1) Mouse Monoclonal Antibody [Clone ID: 6F-H2]

Specifications

Product Data
Clone Name	6F-H2
Applications	ICC/IF, IHC, IP, Simple Western, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen: 1: 400, Immunohistochemistry-Paraffin: 1:400, Simple Western: 1:50, Western Blot: 2 ug/ml, Immunoprecipitation: 2-10 ug/ml lysate, Immunohistochemistry: 1:400
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant WT1 protein (residues 1-181). [Swiss-Prot# P19544]
Formulation	Tris-glycine, 150mM NaCl and 0.05% sodium azide
Purification	IgG purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	Wilms tumor 1
Database Link	NP_077744 Entrez Gene 7490 Human UniProt ID P19544
Background	Wilms?tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and, like retinoblastoma, is observed in both sporadic and inherited forms. The Wilms?tumor locus has been mapped at chromosome 11p13 as a tumor suppressor gene which encodes a DNA binding protein with four zinc fingers and a glutamine-proline rich amino terminus. The Wilms?tumor protein binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators. However, in contrast to Egr transcription factors, WT1 behaves as a transcriptional repressor in transient transfection assays with synthetic promotor constructs.
Synonyms	AWT1; EWS-WT1; GUD; NPHS4; WAGR; WIT-2; WT33
Reference Data
Protein Families	Druggable Genome, Transcription Factors

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