RAG2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
Reactivity	Human, Mouse, Pig, Rabbit, Rat, Bovine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant fragment corresponding to a region within amino acids 271 and 527 of RAG2 (Uniprot ID#P55895)
Formulation	0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Concentration	lot specific
Purification	Purified by antigen-affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	59 kDa
Gene Name	recombination activating gene 2
Database Link	NP_000527 Entrez Gene 19374 MouseEntrez Gene 295953 RatEntrez Gene 5897 Human UniProt ID P55895
Background	This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq]
Synonyms	RAG-2
Note	Seq homology of immunogen across species: Mouse (85%), Pig (90%), Rabbit (91%), Rat (86%), Bovine (91%)
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Primary immunodeficiency

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