PROX1 Mouse Monoclonal Antibody [Clone ID: 5G10]

Specifications

Product Data
Clone Name	5G10
Applications	IHC
Recommend Dilution	IHC on paraffin sections: 1:1000
Reactivity	Chicken, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human Prox1 protein
Formulation	100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	prospero homeobox 1
Database Link	NP_002754 Entrez Gene 19130 MouseEntrez Gene 305066 RatEntrez Gene 5629 Human UniProt ID Q92786
Synonyms	prospero-related homeobox 1; prospero homeobox 1
Note	Protocol for paraffin sections, without antigen retrieval: Deparaffinized and rehydrate sections. Block sections in 0.1% BSA with 0.25% triton-X-100 in 0.1M tris-saline + 5% horse serum for 1 hr at RT. Dilute PROX1 antibody 1:1000 in 0.1% BSA with 0.25% triton-X-100 in 0.1M tris-saline and incubate overnight at 4 C. Wash 2 x 10 min in 0.1% BSA with 0.25% triton-X-100 in 0.1M tris-saline + 1% horse serum Incubate in biotinylated secondary at 1:5000 dilution for 1 hr at RT Wash 2 x 10 min 0.1% BSA with 0.25% triton-X-100 in 0.1M tris-saline + 1% horse serum Incubate in ABC-HRP reagent for 1hr at RT Wash 2 x 10 min 0.1% BSA with 0.25% triton-X-100 in 0.1M tris-saline Incubate in DAB for 6 minutes.
Reference Data
Protein Families	Embryonic stem cells, ES Cell Differentiation/IPS

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