Cyclin B1 (CCNB1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	ELISA: 1:50,000, WB: 1:100 - 1:1,000, IP: 1:100
Reactivity	Human, Mouse, Dog, Hamster, Rat, Chimpanzee
Host	Rabbit
Clonality	Polyclonal
Immunogen	This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 120-131 of Human Cyclin B1 protein.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	cyclin B1
Database Link	NP_114172 Entrez Gene 25203 RatEntrez Gene 268697 MouseEntrez Gene 608420 DogEntrez Gene 891 Human UniProt ID P14635
Synonyms	CCNB
Note	Cyclin B1 (also called CCNB1 and G2/mitotic-specific cyclin B1) is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form a serine/threonine kinase holoenzyme complex called the maturation-promoting factor (MPF). Two alternative transcripts have been found a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. S126 phosphorylation, the site of phosphorylation recognized by this antibody, is involved in an auto-phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	Cell cycle, Oocyte meiosis, p53 signaling pathway, Progesterone-mediated oocyte maturation

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