SGK1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC
Recommend Dilution	ELISA: 1:4,000 - 1:16,000, WB: 1:500 - 1:3,000, IHC: 10 ug/ml to 20 ug/ml
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	SKG-1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 419-431 of human SGK -1.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	serum/glucocorticoid regulated kinase 1
Database Link	NP_001137148 Entrez Gene 20393 MouseEntrez Gene 29517 RatEntrez Gene 6446 Human UniProt ID O00141
Synonyms	SGK
Note	SGK-1 (also called Serine/threonine-protein kinase Sgk1 and Serum/glucocorticoid-regulated kinase 1) is a protein kinase that plays an important role in cellular stress response. SGK1 activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. This kinase mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A and phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, Kv1.3, or EAAT1. SGK1 is localized to the cytoplasm and upon phosphorylation is translocated to the nucleus. The kinase is expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Induction occurs upon exposure to glucocorticoids and by excessive extracellular glucose or TGF-beta, in cultured cells. SGK-1 is regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1. The kinase is ubiquitinated by NEDD4L; which promotes proteasomal degradation.
Reference Data
Protein Families	Druggable Genome, Protein Kinase

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.