GRP94 (HSP90B1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:1000, IP: 1:80
Reactivity	Human, Mouse, Rat, Bovine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to the sequence near the C-terminus of mouse GRP94.
Formulation	PBS pH 7.4, 0.09% azide.
Concentration	lot specific
Purification	Peptide Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	heat shock protein 90kDa beta family member 1
Database Link	NP_003290 Entrez Gene 22027 MouseEntrez Gene 362862 RatEntrez Gene 7184 Human UniProt ID P14625
Background	Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion . Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway Grp94 is a member of the Hsp90 family of stress proteins and shares sequence homology with its cytosolic equivalent, Hsp90 . Both Hsp90 and Grp94 are calcium binding proteins . Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with Hsp90, Grp94 and Hsp90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity .Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. . Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER .
Synonyms	ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1
Note	Detects ~94kDa.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	NOD-like receptor signaling pathway, Pathways in cancer, Prostate cancer

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