C3AR1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3,000; FC: 1:50-1:600
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)EDHETSPLDNSD, corresponding to amino acid residues 276-287 of human C3a anaphylatoxin receptor. 2nd extracellular loop.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	complement component 3a receptor 1
Database Link	NP_004045 Entrez Gene 719 Human UniProt ID Q16581
Background	The innate immune system identifies pathogens by recognizing known elements. This response ultimately leads to the production of proinflammatory substances and the activation of phagocytic neutrophils and macrophages. Activation of the complement system is an important initial even in the response against pathogens. This system includes some thirty different proteins that act together to form a complex. Three different pathways are known to activate the complement. The C3 complement plays a key role in all three pathways leading to the activation of the complement. Produced in the liver, C3 is the most abundant complement protein in the serum. It is composed of two polypeptides, an α and β chain. Cleavage of C3 leads to the production of C3b and C3a. C3a binds to C3a receptor, a G-protein coupled receptor which couples to Gi upon activation. Like all other members of the superfamily, C3a receptor has seven transmembrane domains with an extracellular N-terminus and intracellular C-terminal tail. The receptor has two glycosylation sites, an unusually large extracellular loop with a sulfated tyrosine residue important for ligand recognition and binding. C3a receptor is expressed in cells of myeloid origin like neutrophils, macrophages and dendritic cells to name a few. It is also detected in astrocytes from inflamed brain, endothelial cells and activated human T cells. mRNA of C3a receptor is detected in lung, liver, kidney, brain heart, muscle and testis.
Synonyms	AZ3B; C3AR; HNFAG09
Reference Data
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Complement and coagulation cascades, Neuroactive ligand-receptor interaction

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.