ORAI1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3,000; FC: 1:50-1:600
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)KKQPGQPRPTSK, corresponding to amino acid residues 203-214 of human Orai1. 2nd extracellular loop.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	ORAI calcium release-activated calcium modulator 1
Database Link	NP_116179 Entrez Gene 84876 Human UniProt ID Q96D31
Background	Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.The mechanism controlling intracellular Ca2+ level influx either by the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores, has become of great interest.Recently, several key players of the store operated complex have been identified.Orai1 (also known as CRACM1) acts as the store operated calcium channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor.The formation of functional channels by Orai1 requires the presence of both Orai1 and STIM1 proteins working as a complex that involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum.TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex.In T-cells, Ca2+ entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit.Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-terminus. In addition, two mammalian homologs to Orai1 have been identified; Orai2 and Orai3. All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes.
Synonyms	CRACM1; IMD9; ORAT1; TAM2; TMEM142A
Reference Data
Protein Families	Transmembrane

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