Caspase 8 (CASP8) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, IP, Simple Western, WB
Recommend Dilution	Western Blot: 1:1000-1:2000, Immunohistochemistry: 1:10-1:500, Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Immunoprecipitation: 1:50-1:200, Immunohistochemistry-Paraffin: 1:1000-1:5000, Immunohistochemistry-Frozen: 1:10-1:500, Flow Cytometry, Simple Western
Reactivity	Human, Mouse, Rat, Gerbil
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant catalytically active human Caspase-8 protein was used as immunogen.
Formulation	Store at -20C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	55.4 kDa
Gene Name	caspase 8
Database Link	NP_203519 Entrez Gene 12370 MouseEntrez Gene 64044 RatEntrez Gene 841 Human UniProt ID Q14790
Background	Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer as well as other autoimmune diseases. Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive pro-enzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 and a 12 kDa fragment which is further processed to 10 kDa. The p43 is then cleaved to yield p26 and the release of the active site containing p18.
Synonyms	ALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5
Reference Data
Protein Families	Druggable Genome, Protease
Protein Pathways	Alzheimer's disease, Apoptosis, Huntington's disease, NOD-like receptor signaling pathway, p53 signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Viral myocarditis

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