CXCR4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	Western Blot: 1 - 2 ug/mL, Immunohistochemistry: 1:10 - 1:500, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Immunohistochemistry-Frozen), Immunohistochemistry-Paraffin: 1:10 - 1:500
Reactivity	Human, Mouse, Rat, Sheep
Host	Rabbit
Clonality	Polyclonal
Immunogen	Rabbit anti-CXCR4 polyclonal antibody was raised against a peptide corresponding to amino acids 328-338 of human CXCR4.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	C-X-C motif chemokine receptor 4
Database Link	NP_003458 Entrez Gene 12767 MouseEntrez Gene 60628 RatEntrez Gene 7852 Human UniProt ID P61073
Background	Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors1. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells (2,3). CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2 (4,5). The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1 (6,7). CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells (8). CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types (2). Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells (2,5,10). The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites (10,11).
Synonyms	CD184; D2S201E; FB22; HM89; HSY3RR; LAP-3; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; WHIM
Note	Immunohistochemistry (cryosections): see Nimmagadda etl al (2010) for details. Immunohistochemistry (paraffin): see Zagzag (2005) and others for details.
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane
Protein Pathways	Axon guidance, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Endocytosis, Leukocyte transendothelial migration

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