IKK beta (IKBKB) Mouse Monoclonal Antibody [Clone ID: 10AG2]

Specifications

Product Data
Clone Name	10AG2
Applications	CyTOF-ready, FC, ICC/IF, IHC, Immunoblotting, IP, Simple Western, WB
Recommend Dilution	Immunoprecipitation: 1:10 - 1:500, Simple Western: 1:12.5, Immunohistochemistry-Paraffin: 1:10 - 1:500, Immunocytochemistry/ Immunofluorescence: 2-5 ug/ml, Immunohistochemistry: 1:10 - 1:500, Immunoblotting, CyTOF-ready, Flow Cytometry: 0.25 - 0.5 ug/ 10^6 cells, Western Blot: 2 - 4 ug/ml
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	A full-length human IKKb recombinant protein was used to immunize mice (NP_001547).
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at -20C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	86.564 kDa
Gene Name	inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
Database Link	NP_001547 Entrez Gene 16150 MouseEntrez Gene 3551 Human UniProt ID O14920
Background	NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli. Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo.
Synonyms	IKK-beta; IKK2; IKKB; IMD15; NFKBIKB
Note	Useful in Immunohistochemistry-Paraffin See Page et al.
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Transcription Factors
Protein Pathways	Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus

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