RANKL (TNFSF11) Mouse Monoclonal Antibody [Clone ID: 12A668]

Specifications

Product Data
Clone Name	12A668
Applications	ChIP, ELISA, FC, IA, ICC/IF, IHC, WB
Recommend Dilution	ELISA, Chromatin Immunoprecipitation (ChIP): 1:10-1:500, Flow (Cell Surface), Immunoassay, Immunohistochemistry: 1:10-1:500, Immunohistochemistry-Paraffin: 5 ug/ml, Immunocytochemistry/ Immunofluorescence: 20 ug/ml, Western Blot: 0.5-2 ug/ml
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE was used as immunogen (NP_003692).
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	35 kDa
Gene Name	tumor necrosis factor superfamily member 11
Database Link	NP_003692 Entrez Gene 21943 MouseEntrez Gene 117516 RatEntrez Gene 8600 Human UniProt ID O14788
Background	TRANCE/OPGL/RANKL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells. RANK ligand (RANKL/TRANCE) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. TRANCE/RANKL is also important in T and B-cell maturation. As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis. OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.
Synonyms	CD254; hRANKL2; ODF; OPGL; OPTB2; RANKL; sOdf; TRANCE
Note	Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cytokine-cytokine receptor interaction

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