TLR4 Rat Monoclonal Antibody [Clone ID: MTS510]

Specifications

Product Data
Clone Name	MTS510
Applications	FC, ICC/IF, IP
Recommend Dilution	Immunocytochemistry/ Immunofluorescence, Flow Cytometry: 1-2 ug/10^6 cells, Flow (Cell Surface): 1 - 2 ug / 10^6 cells, Immunoprecipitation: 1:10-1:500
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	This antibody was developed by immunizing rats with the Ba/F3 cell line expressing mouse TLR4 and MD-2 (Akashi et al, 2000).
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	toll like receptor 4
Database Link	NP_612564 Entrez Gene 21898 Mouse UniProt ID O00206
Background	The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling. The mouse TLR4 cDNA codes for a protein consisting of 835 amino acids with approximate molecular weight of 90 kDa.
Synonyms	ARMD10; CD284; TLR-4; TOLL
Note	Immunoprecipitation: Please see Akashi et al (2000) for details
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway

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